ntozier New 2FA plugin is "Two Factor Authenticator" whereas previous one is "Google Authenticator 2FA" - as far as osTicket is concerned, they're completely different plugins. (I know you know this, was just clarifying the previous post 😇 )
@bin360 Yep, you need to enable the new one (and you can tidy up by removing the old one) - once done, all bindings will be reset, so anyone using 2FA will have to re-register.